Table 3.
Mortality of Galleria mellonella after injected with V. cholerae wild type and indole deficient mutant
V. cholerae strains | G. mellonella mortality within 36 h (%) |
---|---|
Mean ± SD | |
Control (indole) | 0 |
Wild type | 63.3 ± 4.7a |
Mutant | 43.0 ± 4.2b |
Mutant + 0.5 mM indole | 49.5 ± 4.9bc |
Mutant + 1.0 mM indole | 56.5 ± 4.9ac |
Mutant + 2.0 mM indole | 63.0 ± 4.2a |
Mutant + 4.0 mM indole | 66.0 ± 0a |
Both wild type and mutant strains were cultured in 5 mL of LB broth at 37 °C for 3 h and the concentration of cells was adjusted in PBS. Fifteen of G. mellonella were injected in each category
The difference in super subscribe indicates the significant difference
In control group, the larvae were injected only with indole at the concentration of 0.5–4 mM