Table 3.
Unique in silico restriction endonuclease digestion patterns (5′-3′) of recA gene of Lactobacillus strains
| Lactobacillus spp. | Restriction endonucleases | |||
|---|---|---|---|---|
| AluI | TaqI | Tru9I | BfaI | |
| L. acidophilus La-14 | –a | – | – | – |
| L. acidophilus NCFM | – | – | – | – |
| L. brevis ATCC 367 | – | 720·313·70·31 | 366·323·192·181·48·24 | – |
| L. brevis KB290 | – | 720·313·100·31 | 366·337·192·181·48·24·16 | – |
| L. buchneri CD034 | – | 501·453·159·22·5 | 535·292·117·87·48·37·24 | – |
| L. buchneri NRRL B-30929 | 453·432·170·63·22 | 501·258·195·159·22·5 | 535·379·117·48·37·24 | – |
| L. casei BL23 | – | – | – | – |
| L. casei str. Zhang | – | 580·242·181·59 | 504·381·110·67 | – |
| L. delbrueckii subsp. bulgaricus ATCC 11842 | 290·241·241·224 | 315·166·136·98·81·72·54·36·15·14·9 | – | – |
| L. delbrueckii subsp. bulgaricus NDO2 | – | – | – | – |
| L. fermentum IFO 3956 | – | 360·215·205·117·70·51·18·14·12 | – | – |
| L. gasseri ATCC 33323 | 425·138·136·132·130·98·36 | – | 300·244·168·87·82·68·67·40·24·15 | – |
| L. helveticus CNRZ32 | – | – | 508·334·87·69·67·33 | – |
| L. helveticus DPC 4571 | 270·261·144·144·97·95·87 | – | – | – |
| L. helveticus H10 | – | – | – | – |
| L. helveticus R0052 | – | 454·435·159·50 | – | – |
| L. kefiranofaciens ZW3 | 366·330·162·136·90·18·8 | 784·228·50·48 | 353·259·184·114·111·41·33·15 | – |
| L. paracasei ATCC 334 | – | – | – | – |
| L. plantarum subsp. plantarum P-8 | 353·256·216·108·72·47·39·28·24 | – | 308·270·258·153·53·43·33·21·4 | – |
| L. plantarum ZJ316 | – | – | – | 725·267·102·25·24 |
| L. reuteri I5007 | 368·268·231·193·29 | – | – | – |
| L. reuteri SD2112 | – | 567·247·228·47 | – | – |
| L. reuteri TD1 | – | – | – | – |
| L. rhamnosus ATCC 53103 | 466·166·129·119·100·73 | 370·266·235·103·40·27·12 | 383·240·216·95·70·49 | 541·422·73·17 |
| L. rhamnosus GG ATCC 53103 | – | – | 535·289·165·40·24 | – |
| L. rhamnosus LOCK900 | 478·339·133·54·49 | 824·124·56·49 | – | – |
| L. ruminis ATCC 27782 | 491·249·174·94·81 | 437·227·220·109·96 | – | – |
| L. sakei subsp. sakei 23 K | – | 372·298·172·150·108·36 | 352·329·219·165·30·24·14·3 | – |
| L. salivarius UCC118 | 420·344·207·63·55·45·12 | – | 535·225·160·117·49·39·21 | – |
| L. sanfranciscensis TMW 1.1304 | 903·74·46·43·42·5 | 266·206·193·157·123·118·50 | 344·184·174·156·138·61·43·10·3 | – |
Additional unique patterns were also recorded for L. fermentum IFO 3956 - Hin1I - 552·189·124·108·89
Symbol (·) indicates RE site in the gene sequences
aNo unique pattern was observed