Figure 3.

Correlation of Regulation by Antisense with Antisense and Protein Levels

(A) The effect of antisense on protein levels (log2-fold change of sfGFP levels between PHO5_T:scr and PHO5_T) versus antisense transcript levels. Data obtained for all conditions from fluorescence microscopy and from tiling arrays are shown. These were obtained using the same strain background and the same growth conditions. Blue circles represent genes not found to be regulated by antisense. Red and green circles indicate genes with regulation by antisense with the latter being subject to an antisense-dependent on/off switch (see Figure 2 and main text). Spearman’s correlation coefficient ρ is indicated in the plot.

(B) As in (A), but with sfGFP intensities of the PHO5_T:scr construct plotted on the x axis.