Figure 2.
MpLRL Is a Growth Regulator Required for Both Dorsal Thallus and Rhizoid Development
(A–D) Phenotypes of plants grown from gemmae transformed with the inducible MpLRLamiRMp160II transgene in inductive conditions compared to wild-type. (A) Wild-type plants with category (C1) phenotype, (B) plants with initiating outgrowth (colored patch) on the dorsal thallus indicative for an outgrowing thallus (C3), (C) plants with secondary thallus formed on the dorsal side (C4, arrowheads indicate meristems: white arrow, primary thallus; black arrow, secondary thallus), and (D) plants with only rhizoid-less callus like tissue developing on the dorsal thallus (C5).
(E and F) Quantification of meristematic phenotypes of MpLRLamiRII-ind-1 grown under inductive conditions. (E) Wild-type and (F) MpLRLamiRII-ind-1.
(G and H) Quantification of meristematic phenotypes of two independent double-transformants of proOsAct:MpLRLres in the MpLRLamiRII-ind-1. Transformant line #1 (G) and transformant line #7 (H). Note the large number of relatively wild-type-like meristems and the small number of the strong, category C5 phenotypes.
(I and J) Fully grown wild-type M. polymorpha thalli. (I) Dorsal view and (J) ventral view are shown.
(K and L) Fully grown M. polymorpha thalli transformed with the proOsAct:MpLRL-SRDX fusion. (K) Dorsal view; (L) ventral view. Note the lack of dorsal-like tissue types in (K) and the lack of rhizoids in (L).
Scale bars, 500 μm. See also Figure S3.