Table 5.
Summary of results found for markers associated with traits of interest at least in two years of study and comparison with sorghum genome
| Locus | Cane Yield | Sugar Content | DArT sequence size (pb) | BLAST algorithm d | Result from alignament with S. bicolor | Identity | Expect | Locus tag | Sorghum chromosome | GenBank ID | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2009 | 2010 | 2011 | 2009 | 2010 | 2011 | |||||||||
| M151 | * | * | 748 | blastn | hypothetical protein | 49/64(77 %) | 0.001 | Sb01g041060 | 1 | XM_002468130.1 | ||||
| M97 | * | * | 456 | m blast | hypothetical protein | 419/459(91 %) | 9.00E-173 | Sb02g004780 | 2 | XM_002461519.1 | ||||
| M41 | * | ** | 263 | blastn | hypothetical protein | 45/58(78 %) | 0.25 | Sb02g028450 | 2 | XM_002460412.1 | ||||
| M91 | * | * | 404 | dm blast | hypothetical protein | 68/68(100 %) | 1.00E-27 | Sb03g004640 | 3 | XM_002457229.1 | ||||
| M64 | * | * | * | 291 | m blast | hypothetical protein | 287/291(99 %) | 6.00E-143 | Sb03g035700 | 3 | XM_002456357.1 | |||
| M78 | * | * | 474 | m blast | hypothetical protein | 79/83(95 %) | 4E-30 | Sb04g025280 | 4 | XM_002452369.1 | ||||
| M125 | * | * | 1048 | dm blast | hypothetical protein | 87/121(72 %) | 9E-08 | Sb05g000890 | 5 | XM_002448838.1 | ||||
| M95 | * | * | 545a | dm blast | hypothetical protein | 122/146(84 %) | 2E-37 | Sb06g014780 | 6 | XM_002447689.1 | ||||
| M50 | * | * | 545a | dm blast | hypothetical protein | 122/146(84 %) | 2E-37 | Sb06g014780 | 6 | XM_002447689.1 | ||||
| M120 | * | * | 457 | m blast | hypothetical protein | 407/435(94 %) | 0 | Sb06g000450 | 6 | XM_002445961.1 | ||||
| M57 | * | *** | 60 | blastn | hypothetical protein | 20/21(95 %) | 0.34 | Sb06g000690 | 6 | XM_002445988.1 | ||||
| M189 | * | * | 355 | blastn | hypothetical protein | 18/18(100 %) | 1.2 | Sb06g025190 | 6 | XM_002448276.1 | ||||
| M46 | * | * | 371b | m blast | hypothetical protein | 119/121(98 %) | 4E-54 | Sb07g020840 | 7 | XM_002444326.1 | ||||
| M30 | * | ** | 384b | m blast | hypothetical protein | 119/121(98 %) | 4E-54 | Sb07g020840 | 7 | XM_002444326.1 | ||||
| M71 | * | * | 425 | blastn | hypothetical protein | 59/73(81 %) | 3.00E-09 | Sb08g022800 | 8 | XM_002443601.1 | ||||
| M58 | ** | * | * | 566 c | m blast | hypothetical protein | 354/371(95 %) | 7E-168 | Sb09g002270 | 9 | XM_002439164.1 | |||
| M54 | ** | * | * | 566 c | m blast | hypothetical protein | 354/371(95 %) | 7E-168 | Sb09g002270 | 9 | XM_002439164.1 | |||
| M168 | * | * | 384 | m blast | hypothetical protein | 368/384(96 %) | 7E-176 | Sb09g015250 | 9 | XM_002440847.1 | ||||
| M59 | * | * | 468 | blastn | hypothetical protein | 23/25(92 %) | 0.13 | Sb09g007900 | 9 | XM_002439437.1 | ||||
| M193 | * | ** | 604 | blastn | hypothetical protein | 18/18(100 %) | 2.1 | Sb09g014225 | 9 | XM_002439535.1 | ||||
| M153 | * | * | 753 | dm blast | hypothetical protein | 85/87(98 %) | 6E-35 | Sb10g006890 | 10 | XM_002437984.1 | ||||
| M14 | * | * | 427 | blastn | hypothetical protein | 23/24(96 %) | 0.43 | Sb10g010770 | 10 | XM_002438218.1 | ||||
| M188 | * | * | * | 578 | blastn | hypothetical protein | 29/34(85 %) | 0.59 | Sb10g023910 | 10 | XM_002437224.1 | |||
| M45 | ** | * | 749 | blastn | alpha kafirin | 79/98(81 %) | 3.00E-18 | unassigned | Y17556.1 | |||||
| M181 | ** | * | 749 | blastn | alpha kafirin | 46/46(100 %) | 5E-16 | unassigned | Y17556.1 | |||||
| M108 | * | * | 488 | m blast | alpha kafirin | 61/62(98 %) | 7E-23 | unassigned | Y17556.1 | |||||
| M173 | * | * | * | 662 | blastn | alpha kafirin | 39/41(95 %) | 5E-09 | unassigned | Y17556.1 | ||||
| M5 | * | * | na | |||||||||||
| M86 | * | * | na | |||||||||||
| M32 | * | * | na | |||||||||||
| M198 | * | * | na | |||||||||||
| M197 | ** | * | na | |||||||||||
| M203 | * | * | na | |||||||||||
na not available sequence
FDR P-values: * p < 0.01; ** p < 0.001; and *** p < 0.0001
a, b, cindicate same nucleotide sequence for two diferent DArT marker
dmegablast (m blast), discontiduous mega blast (dm blast) or blastn
Data in bold = more significant alignment i.e. larger sequence size with high identity and lower Expected value