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. 2016 Jun 10;53(5):2463–2475. doi: 10.1007/s13197-016-2231-y

Table 3.

Tolerance of biological barriers in stimulated GI tract and cell surface characteristics of isolated Lactobacillus spp.

pH (log CFU/ml) Bile concentration (log CFU/ml) Autoaggregation* (%) Coaggregation* (%)
Isolates pH 7.0 pH 3.0 pH 2.0 0.5 % 1 % 2 % 3 h 24 h 3 h 24 h
L1 8.47 ± 0.17b 7.32 ± 0.12a 7.04 ± 0.07a 8.35 ± 0.09b 8.30 ± 0.05b 7.82 ± 0.21a 13.35 ± 0.04klmn 59.35 ± 0.39 5.84 ± 0.04 50.19 ± 0.09
L5 8.72 ± 0.05c 8.43 ± 0.06b 8.28 ± 0.05a 8.64 ± 0.08b 8.60 ± 0.09b 8.16 ± 0.16a 10.75 ± 0.04hijkl 47.42 ± 0.03 16.45 ± 0.03 42.76 ± 0.04
L6 7.98 ± 0.10a 7.86 ± 0.09a 7.81 ± 0.12a 8.07 ± 0.05c 8.03 ± 0.03c 8.01 ± 0.08c 7.65 ± 0.04abdefghi 41.39 ± 0.05 13.35 ± 0.03 45.57 ± 0.03
L9 8.93 ± 0.05b 8.70 ± 0.08b 8.45 ± 0.13a 8.84 ± 0.06b 8.80 ± 0.02b 8.37 ± 0.10a 40.61 ± 0.02p 72.94 ± 0.04 15.44 ± 0.04 61.68 ± 0.02
L11 7.72 ± 0.06c 7.57 ± 0.05b 7.01 ± 0.03a 7.66 ± 0.05b 7.61 ± 0.05b 7.26 ± 0.16a 9.15 ± 0.02fghijk 50.86 ± 0.02d 18.23 ± 0.02 42.21 ± 0.18
L14 7.93 ± 0.05c 7.22 ± 0.10b 6.94 ± 0.05a 7.82 ± 0.05bc 7.67 ± 0.03ab 7.57 ± 0.09a 12.19 ± 0.02ijklm 65.07 ± 0.06f 19.84 ± 0.02 61.13 ± 0.03
L16 8.54 ± 0.04c 8.25 ± 0.05b 8.07 ± 0.05a 8.46 ± 0.06bc 8.40 ± 0.06b 8.01 ± 0.03a 16.10 ± 0.02mno 68.22 ± 0.03 23.43 ± 0.2 72.11 ± 0.01
L19 6.10 ± 0.07b 5.76 ± 0.19a 5.19 ± 0.14a 6.05 ± 0.07b 5.91 ± 0.02b 5.32 ± 0.11a 8.23 ± 0.03efghi 54.09 ± 0.08 15.43 ± 0.02 56.21 ± 0.02
L21 8.40 ± 0.04b 8.18 ± 0.06a 8.06 ± 0.04a 8.34 ± 0.05b 8.32 ± 0.06b 7.76 ± 0.14a 8.67 ± 0.02efghi 72.05 ± 0.03 22.04 ± 0.02 68.62 ± 0.02
L22 8.60 ± 0.04a 8.52 ± 0.04a 8.49 ± 0.07a 8.79 ± 0.06b 8.75 ± 0.07b 8.71 ± 0.06b 18.74 ± 0.02o 71.05 ± 0.02g 16.23 ± 0.02 66.21 ± 0.02
L23 8.17 ± 0.05b 8.06 ± 0.03b 7.93 ± 0.05a 8.07 ± 0.05b 8.03 ± 0.04b 7.48 ± 0.19a 11.94 ± 0.02ijklm 51.83 ± 0.02 14.16 ± 0.02 45.21 ± 0.02
L24 9.20 ± 0.36b 8.68 ± 0.19ab 8.35 ± 0.31a 9.11 ± 0.36ab 9.08 ± 0.03ab 8.58 ± 0.09a 13.18 ± 0.02jklmn 52.72 ± 0.03 16.14 ± 0.02 67.21 ± 0.02
L26 7.89 ± 0.05b 7.72 ± 0.05a 7.57 ± 0.06a 7.80 ± 0.01b 7.76 ± 0.02b 7.22 ± 0.10a 9.53 ± 0.03ghijk 51.23 ± 0.02e 3.10 ± 0.01 11.02 ± 0.02
L28 9.52 ± 0.05c 9.21 ± 0.05b 9.07 ± 0.03a 9.44 ± 0.03b 9.40 ± 0.03b 8.79 ± 0.21a 10.23 ± 0.02hijk 48.04 ± 0.02 19.34 ± 0.03 39.21 ± 002
L29 8.72 ± 0.04c 8.58 ± 0.06b 8.33 ± 0.06a 8.66 ± 0.05b 8.63 ± 0.05b 8.34 ± 0.15a 9.32 ± 0.23fghijk 51.04 ± 0.02de 28.53 ± 0.02 61.21 ± 0.02
L31 7.82 ± 0.05b 7.66 ± 0.05b 7.25 ± 0.14a 7.76 ± 0.08b 7.70 ± 0.10b 7.04 ± 0.11a 11.53 ± 0.02ijklm 71.11 ± 0.02g 29.55 ± 0.02 67.21 ± 0.02
L34 8.08 ± 0.05c 7.89 ± 0.11b 7.62 ± 0.05a 7.99 ± 0.01b 7.93 ± 0.02b 7.58 ± 0.12a 11.76 ± 0.03ijklm 57.23 ± 0.02 18.32 ± 0.02 49.71 ± 0.01
L36 8.07 ± 0.05c 7.75 ± 0.12b 6.81 ± 0.16a 7.96 ± 0.10b 7.92 ± 0.06b 7.56 ± 0.12a 11.84 ± 0.01ijklm 51.35 ± 0.04e 13.73 ± 0.02 68.12 ± 0.02
L38 9.53 ± 0.07c 8.71 ± 0.03b 8.05 ± 0.04a 9.46 ± 0.09b 9.40 ± 0.10b 8.36 ± 0.14a 12.08 ± 0.03ijklm 53.35 ± 0.03 18.06 ± 0.03 51.22 ± 0.02
L40 7.87 ± 0.05b 7.49 ± 0.18a 7.21 ± 0.10a 7.78 ± 0.07b 7.73 ± 0.06b 7.34 ± 0.11a 17.05 ± 0.03no 71.07 ± 0.03g 13.47 ± 0.02 65.61 ± 0.01
P1 8.09 ± 0.02c 7.85 ± 0.04b 6.84 ± 0.03a 7.91 ± 0.11b 7.84 ± 0.04b 7.49 ± 0.12a 15.07 ± 0.06lmno 65.02 ± 0.03f 12.02 ± 0.03 61.03 ± 0.03
P2 9.21 ± 0.36c 8.64 ± 0.08b 7.89 ± 0.02a 9.07 ± 0.36b 9.32 ± 0.03b 8.16 ± 0.01a 18.02 ± 0.02o 69.02 ± 0.03 21.02 ± 0.03 68.03 ± 0.03

Values represented as mean ± SD; for each row, different subscripts lowercase letters indicate significantly different at p < 0.05, as measured by 2-sided Tukey’s HSD between bile %, 0.5, 1, 2, control and between pH 2, pH 3 and pH 7

P1 and P2 (L. casei Shirota and L. rhamnosus) are probiotic reference strains

*Values represented as mean ± SD; for 3 h column, different subscripts lowercase letters indicate significantly different at p < 0.00001; for column 24 h, similar superscript letters are not significantly differ while remaining unsuperscript are significantly differ at p < 0.00001, as measured by 2-sided Tukey’s HSD between different isolates