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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biomaterials. 2016 Jun 9;101:321–340. doi: 10.1016/j.biomaterials.2016.06.018

Fig. 4.

Fig. 4

Assess the biocompatibility of GDNPs in vitro. (A) MTT cell proliferation assay was used to assess the potential toxicity of GDNPs 2 in colon-26 and RAW 264.7 macrophage-like cell lines. (B) Barrier function assay was used to determine the influence of GDNPs 2 to the barrier function on caco2-BBE monolayer. (C) At the end of barrier function assay, PBS treated cells were stained with phalloidin-TRITC. Scale Bar=20 μm. (D) At the end of barrier function assay, GDNPs 2 (100 μg/mL) treated cells were stained with phalloidin-TRITC. Scale Bar=20 μm. (E) Cytotoxicity effect of GDNPs 2 on colon-26 cells and RAW 264.7 mouse macrophages after 24 h incubation were measured by FACS. Colon-26 and RAW 264.7 cells were incubated with indicated concentrations of GDNPs 2 for 24 h and then stained with Annexin-V/PI to detect the cell death. Lower left, viable cells (Annexin-V−/PI−); lower right, early apoptotic cells (Annexin-V+/PI−); upper left, necrotic cells (Annexin-V−/PI+); upper right, late apoptotic cells (Annexin-V+/PI+). (n=3).