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. Author manuscript; available in PMC: 2016 Jun 26.
Published in final edited form as: Oncogene. 2013 Nov 4;33(45):5238–5250. doi: 10.1038/onc.2013.467

Figure 5.

Figure 5

Induction of hallmarks of mitochondrial retrograde signaling in mtDNA-reduced MCF10A and MCF7 cells. (a) Calcineurin activity in parental, mtDNA-reduced and reverted MCF10A and MCF7 cells. (b, c) Immunoblot showing IGF1 receptor, phospho-Akt (Ser473) and total Akt expression levels in total cell extracts (50 μg) of parental, mtDNA-reduced and reverted MCF10A (b) and MCF7 (c) cells. Beta actin was used to assess equal protein loading. (d, e) Percentage of apoptosis in parental, EtBr-treated mtDNA-reduced and Tfam-silenced mtDNA-reduced MCF10A and MCF7 cells treated with either DMSO or picropodophyllin.