Skip to main content
. Author manuscript; available in PMC: 2016 Jun 26.
Published in final edited form as: Neuron. 2007 Jun 21;54(6):873–888. doi: 10.1016/j.neuron.2007.05.024

Figure 2. Interneurons from neocortex of Dlx1−/−;Dlx2+/− compound mutants develop neuronal processes with fewer branches.

Figure 2

(A) Experimental assay used to characterize the morphology of interneurons.

(B, C) Examples of interneurons isolated from P0 neocortex and immunostained with anti-GFP after 5 DIV. (B′, C′) are drawings of representative neurons.

(D, E) Quantification of axonal and dendritic morphology from control (gray bars) and Dlx1−/−;Dlx2+/− (black bars) interneurons after 5 DIV. Student’s t-test revealed significantly decreases in the numbers of dendritic and axonal branches in mutant cells (dendrites: mutant: 16.6 ± 1; control: 21.8 ± 1.2, p < 0.01; axons: mutant: 12 ± 0.8; control: 17.3 ± 1.6, p < 0.01; n = 30). ** p < 0.01.

Scale bar = 100 μm (B,C).