Skip to main content
. Author manuscript; available in PMC: 2016 Jun 26.
Published in final edited form as: Neuron. 2007 Jun 21;54(6):873–888. doi: 10.1016/j.neuron.2007.05.024

Figure 5. Analysis of PAK3 expression in the mouse telencephalon.

Figure 5

(A–D) RNA in situ hybridization for PAK3 in coronal sections through the telencephalon of wild-type (A–D) and Dlx1/2−/− mutant (A′–C′) embryos at E11.5, E14.5, and P0. Note the absence of detectable PAK3 expression in the MGE SVZ of wild-type embryos, and increased expression in the MGE SVZ of Dlx1/2−/− mutants (arrowheads in A′–C′). (D) PAK3 expression in wild-type postnatal (P7) hippocampus. Black arrows point to PAK3 expression in hippocampal layers that contain interneurons.

(E–G) Immunofluorescent staining for PAK3 protein in cultures from wild-type E15.5 MGE after 3, 5 and 7 DIV.

(H) Immunoblot analysis of PAK3 from cell lysates of MGE cultures after 3, 5 and 7 DIV. Scale bars = 500 μm (A,B,C,D) and 50 μm (E–G).