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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Methods. 2016 Apr 12;103:34–48. doi: 10.1016/j.ymeth.2016.04.002

Figure 7. Comparison of in vitro folded 5S rRNA reactivity maps from different amounts of starting RNA.

Figure 7

The same in vitro SHAPE-Seq experiment was performed using either 1, 5, 10, or 20 pmol of starting RNA (RMDB: 5SRRNA_1M7_0009). As expected, all of the reactivity maps are very similar, although there is some disagreement near positions 102–104 (right inset). However, these differences do not show a trend with increasing/decreasing starting RNA level and are thus likely experimental noise.