Table 1.
Primary Antibody Details
Antibody | Species | Concentration | Company | Catalogue # | Antigen | Staining Characteristic |
---|---|---|---|---|---|---|
Tuj1 | Mouse | 1:300 | Covance Emeryville, CA | MMS-435P | rat brain beta-tubulin III | all neurons |
Beta-Tubulin IV | Mouse | 1:1000 | Sigma St. Louis, MO | T7941 | c-terminal synthetic peptide beta-tubulin IV | respiratory cilia |
hNF | Mouse | 1:50 | Dako North America, Inc. Carpinteria, CA | 2F11 | neurofilament from adult human brain (70KD) | trigeminal axons |
OMP | Goat | 1:100 | Santa Cruz Biotechnology, Inc. Dallas, TX | SC-49070 | rodent olfactory marker protein | mature olfactory neurons |
p75 (NTR) | Mouse | 1:50 | Millipore Billerica, MA | MAB365 | protein isolated from plasma membrane of PC-12 cells | perineurium and ensheathing cells |
PGP9.5 | Rabbit | 1:1000 | Cedarlane Burlington, NC | RA95101 | human brain protein gene product 9.5 | all neurons |
S100 | Rabbit | 1:100 | Dako North America, Inc. Carpinteria, CA | Z0311 | S100 protein from cow extracts | neurons, ensheathing cells, some gland tissue |