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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Pediatr Surg. 2016 Mar 2;51(6):995–1000. doi: 10.1016/j.jpedsurg.2016.02.074

Figure 2. Light microscopy of human intestinal epithelial cultures.

Figure 2

A: A less differentiated monolayer of epithelial cells (Passage 4, Day 3). B: A more differentiated monolayer of epithelial cells (Passage 4, Day 3). C: A less differentiated monolayer of epithelial cells prior to transforming cells into a more differentiated monolayer of cells. D: A more differentiated monolayer generated from supporting monolayers with ENRYG-50Wnt3A and ENRY-DAPT-5Wnt3A. E: Spheroids (Passage 8, Day 5) generated from monolayers cells under light microscopy at 25x magnification. F: Enteroids (Passage 9, Day 16) generated by supporting spheroids with ENRYG-50Wnt3A and ENRY-DAPT-5Wnt3A under light microscopy at 25x magnification.