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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Methods. 2016 Mar 10;103:167–174. doi: 10.1016/j.ymeth.2016.03.003

Fig. 2.

Fig. 2

Commonly used 2′-ribose modified nucleotides in aptamer selection experiments. The mutated T7 (Y639F) RNA polymerase is able to easily incorporate the 2′OH and 2′F modifications during selection, while the 2′Ome modification is normally incorporated synthetically after selection has occurred. (Modified nucleotides and aptamer libraries can be purchased from TriLink BioTechnologies, Inc. – http://www.trilinkbiotech.com/.)