Figure 1.

Expression of EDC genes in Mast cell-deficient mice. A, RNA was isolated from the ear skin of WT and Kit ^W-sh/W-sh mice. Expression of the indicated genes was determined by quantitative PCR analysis of skin RNA. In mast cell reconstitution experiments, bone marrow derived mast cells were adoptively transferred via subcutaneous injection of 10⁶ cells in the shaved dorsal skin of Kit^W-sh/W-sh mice. After 6 weeks, skin was collected for analysis of EDC gene expression. B, Transepidermal water loss (TEWL) of shaved skin from WT and Kit^W-sh/W-sh mice. C, RNA was isolated from the ear skin of WT and Cpa3-Cre mutant mice. D, Immunoblot analysis of ear tissue from WT and Kit^W-sh/W-sh mice for the indicated proteins. E, Densitometry of immunoblot analysis in (D). Quantitative PCR analysis was performed for gene expression from skin tissues. Data shown represent the mean ± SEM of 3 to 7 mice per group. *p < 0.05.