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Mast cell deficiency decreases skin barrier function. The shaved backs of WT and Kit W-sh/W-sh mice were treated with OVA-Alexa 647 for 24h. Uptake of OVA was assessed by measuring the percentage of DCs (CD11c+ MHC-IIhi) that stained positive for Alexa 647 fluorescence. Dot plots (A) are representative from 2 independent experiments with similar results. Bar graph (B) represents the mean ± SEM of the percent positive cells. *Significantly different from WT, p < 0.05.
