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. 2016 Jun 27;6:70. doi: 10.3389/fcimb.2016.00070

Figure 1.

Figure 1

Coxiella burnetii modulates an inflammatory response of pDCs. (A) Flow cytometer graphs showing the gating strategy to study pDCs. (B) CCR7 and CD86 expression of isolated pDCs stimulated (C. burnetii) or not (control) with heat-inactivated C. burnetii or CpG-A (10 μg/ml) during 24 h were visualized (left panel) and quantified (right panel) by flow cytometer. (C) A microarray approach allowed to show the degree of the variation of gene expression (C, left panel), the transcriptional signature (C, right panel) and the modulated genes of pDCs stimulated (C. burnetii) or not (control) with heat-inactivated C. burnetii during 8 h. The microarray was conducted with three biological replicates. (D) Representative graph evaluating the fold change of IL-6, IL-1b, IL-15, and CD40 genes using the microarray or the qRT-PCR approach were effected and statistically analyzed on the basis of the Ct-values. The results are the means ± SD of three independent experiments. **p < 0.01 and ***p < 0.001.