Figure 1.

P-STAT immunohistochemistry in coronal sections of mouse area postrema. After 45 min CNTF treatment, P-STAT3 (A), P-STAT1 (B), and P-STAT5 (C) immunoreactivity was detected in the nucleus of many cells of the area postrema (AP). Insets: the area postrema of control mice processed for immunohistochemistry against the three P-STAT isoforms. Double-staining and confocal microscopy experiments in a CNTF-treated mouse (D–F): P-STAT3 staining is ubiquitous and is also found in the funiculus separans (fs), whereas P-STAT1 staining is detected in a smaller number of cells, most of which lie in the central portion of the area postrema. At higher magnification, the majority of P-STAT3-positive cells also express P-STAT1 (G–I, arrows) or P-STAT5 (J–L), arrows. Panels (G–I) are enlargements of the areas framed in Panels (D–F), respectively. Bar: (A–F), 120 μm; insets of (A–C), 300 μm; (G–I), 25 μm; (J–L), 18 μm.