Figure 3.

Phenotypic characterization of CNTF-responsive neurons in the mouse area postrema. Double-staining and confocal microscopy experiments showed that in mice treated with CNTF for 45 min a few P-STAT3-positive cells were positive for dopamine-beta-hydroxylase (DBH; A–C, arrows) or tryptophan hydroxylase (TPH; D–F, arrows). In contrast, glutamic acid decarboxylase 67 (GAD67; G–I, arrowheads) and choline acetyltransferase (ChAT; J–L, arrowheads) expressing neurons never co-localized with P-STAT3. In a mouse treated with CNTF for 80 min (M–P), some P-STAT3-positive cells also expressed c-Fos (arrows), whereas the P-STAT3 HuC/D-positive neurons did not (arrowheads). Bar: (A–C), 10 μm; (D–L), 12 μm; (M–P), 18 μm.