Figure 5.

c-Fos activation in brainstem cholinergic neurons by CNTF. Double-staining and confocal microscopy experiments after 120 min CNTF treatment showed c-Fos expression (B) in cells of the area postrema (AP), solitary tract nucleus (NTS) and dorsal motor nucleus of the vagus (DMX), depicted by immunofluorescent detection of choline acetyltransferase (ChAT,A), the marker of cholinergic pre-ganglionic vagal neurons. Panels (D–F) are the enlargements of the corresponding areas framed in (A–C), showing two large DMX cholinergic neurons expressing c-Fos (arrows). Some small cholinergic neurons of the NTS were also c-Fos-positive (G–I, arrows). Sol, solitary tract. Bar: (A–C), 50 μm; (D–F), 10 μm; (G–I), 25 μm.