Table 1.
Primary antibodies used in this study.
| Description | Marker | Host/isotype | IHC | IF | Manufacturer |
|---|---|---|---|---|---|
| Signaling | Anti-phospho-specific-(Tyr705)-STAT3 | Rabbit/IgG | 1:1000 | 1:700 | 9131, Cell Signaling Technology Inc. (Beverly, MA, USA) |
| Goat/IgG | 1:1000 | 1:700 | Sc-7993, Santa Cruz Biotech. (Santa Cruz, CA, USA) | ||
| Anti-phospho-specific-(Tyr701)-STAT1 | Rabbit/IgG | 1:1000 | 1:700 | 9167, Cell Signaling Technology Inc. | |
| Anti-phospho-specific-(Tyr694)-STAT5 | Rabbit/IgG | 1:1000 | 1:700 | 9314, Cell Signaling Technology Inc | |
| Cell Marker | Choline Acetyltransferase (ChAT) | Rabbit/IgG | 1:1000 | AB143, Merk Millipore (Darmstadt, Germania) | |
| Dopamine β Hydroxylase (DBH) | Rabbit/IgG | 1:500 | PA5-34664, Thermo Fisher Scientific (Waltham, Massachusetts, USA) | ||
| Glial Fibrillary Acidic Protein (GFAP) | Mouse/IgG | 1:1000 | G3893, Sigma-Aldrich (St Louis, MO, USA) | ||
| Glutamic acid decarboxylase 67 (GAD67) | Mouse/IgG | 1:800 | MAB 5406, Merk Millipore | ||
| Human Neuronal Protein (HuC/D) | Mouse/IgG | 1:50 | A21271, Life technologies (Carlsbad, CA, USA) | ||
| Nestin | Mouse/IgG | 1:300 | MAB353, Merk Millipore | ||
| Tryptophan hydroxylase 2 (TPH2) | Rabbit/IgG | 1:700 | 51124, Cell Signaling Technology Inc. | ||
| Vimentin | Goat/IgG | 1:300 | sc-7557, Santa Cruz Biotech. | ||
| Activity marker | c-Fos | Goat/IgG | 1:5000 | 1:4000 | sc-52-G, Santa Cruz Biotech. |
| Anti-CNTF | CNTF | Goat/IgG | 1:100 | 1:50 | AF-557-NA, R&D Systems (Minneapolis, MN, USA) |