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. 2016 Jun 13;2016:7079487. doi: 10.1155/2016/7079487

Figure 2.

Figure 2

SB-induced renoprotection against CI-AKI involves the PI3K/Akt/Nrf2 pathway. (a) A marked reduction in ΔScr was induced by SB in the rats 24 h after intravenous injection of 10 mL/kg iohexol (350). This effect of SB was abolished by treatment with a specific inhibitor of PI3K (wortmannin) and an activator of Nrf2 (SFN) could mimic the effect of SB. (b, d) Representative photomicrographs of tubular cell injury in rat kidney tissue sections and quantitative analysis of histologic scoring (HE staining). SB significantly attenuated renal tubular injury 24 h after injection of iohexol. This effect of SB was abolished by treatment with wortmannin, and SFN could mimic the effect of SB. (c, e) Representative photomicrographs of immunohistochemical staining for TUNEL in renal sections and quantitative analysis of TUNEL-positive cells. The TUNEL-positive staining was localized in nuclei. SB significantly decreased the number of TUNEL-positive cells in rat kidney tissue sections 24 h after the injection of iohexol. This effect of SB was inhibited by treatment with wortmannin and SFN could mimic the effect of SB. Original magnification (d, e): ×200. ∗ p < 0.05 and ∗∗ p < 0.01 versus the CM group; # p < 0.05 and ## p < 0.01 versus the SB + CM group; n = 8. The values shown are the mean ± SD.