Figure 2.

Expression of cellular proteins in villus cells from in vivo ECwt-infected mice treated with PGZ. (a) Mice (n = 3 per experiment) were infected or not with ECwt and then treated or not with PGZ. Villi isolated from mice were treated for immunochemistry analysis using primary antibodies against rotavirus structural proteins (SP) and secondary HRP-conjugated antibodies and AEC as a substrate. The same slides were subjected to immunofluorescence analysis using the indicated primary antibodies (Ab) and secondary FITC-conjugated antibodies. Representative micrographs are shown. (b) Fluorescence of cells positive to the cellular proteins indicated was measured in randomly chosen triplicate fields per slide using the ImageJ program, and the corrected total cell fluorescence (CTCF) values converted into percentage values are shown as mean (± SD) from three independent experiments performed in duplicate.