Fig. 2. 17β-estradiol (E2) concentration-dependent cell proliferation in Mp- and Fp-BMSCs by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells were grown in steroid-free medium supplemented with different concentrations (0, 10-6, 10-8, 10-10, 10-12, and 10-14 M) of E2 for 14 days. The proliferation rates were quantified by measuring the absorbance at 540 nm. The black bars represent Mp-BMSCs, and the white bars represent Fp-BMSCs. The different superscript letters represent significant (p < 0.05) differences among the five replicates.
