Table. Primers used in cDNA cloning, expression and site-directed mutagenesis of the vacuolar serine protease of Fusarium proliferatum.
| Name | Nucleotide sequence in 5' to 3'-end orientation |
|---|---|
| VSP-F-1 | 5'-25AA(G/A)A(A/G)(C/T)GC(T/C)CC(T/C/A)TGGGG41-3' |
| VSP-R-1 | 5'-761CC(A/G)GC(A/G)AT(A/G)TG(A/G/T)GG(A/G)(A/G/T)A(A/C/G)GCCAT739-3' |
| FuVSP-GSP1 | 5'-186GGCAGGCCCTCGAAGTCGACGT164-3' (For 5' race) |
| FuVSP-GSP2 | 5'-661ATCTTCGCTCCCGGTCTGAACATT684-3' (For 3' race) |
| AP | 5'-GGCCACGCGTCGACTAGTACT-(dT)16-3' |
| AUAP | 5'-GGCCACGCGTCGACTAGTAC-3' |
| AAP | 5'-GGCCACGCGTCGACTAGTACGGGIIGGGIIGGIIG-3' |
| FuVSP-5' Sma I | 5'-TCCCCCGGGG10GACGGCGAGACCGAGCGACAGGC32-3' |
| FuVSP-3' Hind III | 5'-CCCAAGCTTTTA969GTAGCTACCAGCCTCAACAATCTTCTT943-3' |
| FVSP K88A-f | 5'-256GGTACTGTCGCCGGTGCGAAGTACGGTGTTGC287-3' |
| FVSP K88A-r | 5'-287GCAACACCGTACTTCGCACCGGCGACAGTACC256-3' |