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. 2016 Jun 6;37(5):428–443. doi: 10.1016/j.devcel.2016.05.003

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© 2016 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Altered CCSs in Tac-μHD-Transfected HeLa 1.E Cells

(A–E) Deconvolved maximal projections of HeLa clone 1.E cells transfected with Tac-μHD and stained with the indicated antibody combinations. Transfected cells (blue outline), misrecruited juxtanuclear EPS15 and/or HRB (arrowheads), and altered pioneer/CLASP distributions (yellow arrows) are indicated. Sequestration is dose dependent; residual HRB-positive enlarged CCSs (white arrow) in a low Tac-μHD expresser (asterisk) are indicated in (A). Scale bar, 10 μm.

(F–F″) Representative deconvolved maximal projection of GFP-FYVE-μHD transfected (green outline) HeLa 1.E cells stained with the indicated antibodies. Scale bar, 10 μm.