Skip to main content
. 2016 Jun 6;37(5):428–443. doi: 10.1016/j.devcel.2016.05.003

Figure 7.

Figure 7

Transient Higher-Order Complexes to Load Active AP-2 at Growing Assembly Zones

(A and B) Schematic models for edge effects on vectorial CCS initiation. The DPF code (A) will have consequences for the formation and stability of Eps15⋅Fcho1/2, Eps15⋅AP-2, and Eps15⋅Fcho1/2⋅AP-2 nanoclusters. Because of the higher apparent affinity of the Fcho μHD for the DPFs in Eps15/R, formation of Eps15⋅Fcho1/2 will be favored at inchoate sites of CCS assembly.

(C–E) Representative deep-etch electron microscopic image views of adherent ventral cell membranes of HeLa SS6 cells immunogold labeled for FCHO2 with 18 nm colloidal gold (arrows). Proteins assembled around the perimeter (chartreuse) of characteristic polyhedrally assembled clathrin lattice (purple) are pseudo-colored (C). Scale bar, 100 nm.