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. 2016 May 3;5(7):449–458. doi: 10.1016/j.molmet.2016.04.007

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© 2016 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Ghrelin activates an intracellular calcium response as measured by GCaMP6 in intact islets. A: Calcium responses were measured over time in intact islets using the genetically encoded calcium sensor GCaMP6 expressed selectively in delta cells (Sst-Cre × LSL-GCaMP6). Calcium traces of two representative cells in response to changes in glucose concentration and stimulation with 100 nM ghrelin or 30 mM KCl, as indicated. Thumbnails of the islet at the indicated time points in each graph are given, with the top and bottom region of interest corresponding to the top and bottom trace, respectively. Movie of the full calcium trace for this figure is included as Supplemental Video 1.