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. 2016 Apr 22;5(7):527–537. doi: 10.1016/j.molmet.2016.04.004

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

G0S2 overexpression in human primary myotubes inhibits lipolysis and FA oxidation. Representative blots (A) and quantification (B) of G0S2 and ATGL protein content measured in control myotubes (Ad-GFP) and myotubes overexpressing G0S2 (Ad-G0S2) (n = 8). (C) ATGL enzyme activity in control myotubes (Ad-GFP) and myotubes overexpressing G0S2 (Ad-G0S2) (n = 3). Pulse-Chase studies using [1–¹⁴C] oleate were performed to determine the rate of (D) incorporation of radiolabeled oleate into TAG (Ad-GFP = 41 ± 11 nmol/3 h/mg protein), (E) FA release (Ad-GFP = 26.3 ± 2.5 nmol/3 h/mg protein), and (F) oleate oxidation (Ad-GFP = 2.74 ± 0.19 nmol/3 h/mg protein) in control myotubes (Ad-GFP) and myotubes overexpressing G0S2 (Ad-G0S2) (n = 6). *p < 0.05, ***p < 0.001 versus Ad-GFP.