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. 2016 Apr 22;5(7):527–537. doi: 10.1016/j.molmet.2016.04.004

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

G0S2 knockdown increases FA oxidation and induces CER accumulation in skeletal muscle in vivo. (A) IMTG content was measured in control (shNT) and G0S2 silenced (shG0S2) mouse tibialis anterior homogenate (n = 7). (B) Palmitate oxidation rate was measured using [1–¹⁴C] palmitate in control (shNT) and G0S2 silenced (shG0S2) muscle homogenates. Palmitate oxidation (i.e. CO2), acid soluble metabolites accumulation (i.e. ASMs) and total oxidation (i.e. the sum of CO2 release and ASMs accumulation, shNT = 965 ± 126 nmol/h/g tissue) were measured (n = 6). (C) PDK4 protein content was quantified by western blot (n = 6). (D) Ceramide (CER) subspecies content (n = 7), and (E) insulin-stimulated glucose uptake in control (shNT) and G0S2 knockdown (shG0S2) muscles (shNT = 13 ± 3 μmol/min/100 g tissue). (n = 6). *p < 0.05 versus shNT.