Figure 2.

Wortmannin amplifies exocytosis downstream of Ca²⁺entry in to the beta cell. A, (left) Representative capacitance recordings from mouse beta cells treated with DMSO (0.1%) (grey lines) or wortmannin (100 nM) (black lines). (right) Average cumulative capacitance response during each depolarization step is shown. B, shows the same as A, but in beta cell from non-diabetic human donors. C, (left) Representative voltage-dependent Ca²⁺ channel recordings are shown. (right) Average cumulative voltage-dependent Ca²⁺ channel responses during each voltage-step was measured in mouse beta cells treated with DMSO (white circles) or wortmannin (100 nM) (black circles). D, Representative responses to the glucose-stimulated increase in the Fura Red-AM ratio in mouse islets treated with DMSO or wortmannin (100 nM) are shown. (right) The average ratio of the glucose peak/baseline response is shown. *-p < 0.05.