Figure S4.

Adult α-cells converted from β-cells. [MIP-rtTA; TRE-Cre; Rosa26-Tomato; PANIC-ATTAC] mice were subjected to 1-week of doxycycline diet and 1-week washout. Subsequently, mice were subjected to no challenge, an ATTAC challenge, multiparity, or high-fat diet-induced obesity. Pancreas sections were subjected to immunofluorescence of tdTomato and glucagon. The examined islets of individual mice are plotted as circles, which area represents the total number of Tomato⁺ (A) or glucagon⁺ (B) cells in the islet, and the center position marks the percentage of Tomato⁺glucagon⁺ (yellow) cells in the corresponding population.