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Rapid washout of doxycycline-induced Cre expression in pancreatic α-cells. Immunofluorescence of Cre recombinase on pancreas sections from [PPG-rtTA; TRE-Cre] mice after 1-week doxycycline diet (left) or after 2-week doxycycline diet and 1-week washout (right). Top panels: Cre (red) signal only. Bottom panels: Cre merged with insulin (green) and DAPI (blue) signals. Arrowheads: the nuclear Cre signal co-localized with DAPI (purple). Scale bar: 100 μm.
