Figure S7.

The contribution of α-cells to adult β-cells. [PPG-rtTA; TRE-Cre; Rosa26-Tomato; PANIC-ATTAC] mice were subjected to 2-week doxycycline diet followed by 1-week washout. Subsequently, mice were subjected to no challenge, an ATTAC challenge, multiparity, or diet-induced obesity. The ATTAC-challenged mice were examined at 10 or 5 weeks after dimerizer administration. (A and B) Fed blood glucose of ATTAC-challenged mice 2 and 10 weeks (A) or 2 and 5 weeks (B) after dimerizer administration. Dots connected by line represent individual mice. *P < 0.05, **P < 0.01 by paired t-test. (C) Fed body weight of male mice before and after 15 weeks of high-fat diet regimen. Dots connected by line represent individual mice. **P < 0.01 by paired t-test. (D) Mouse pancreas sections were subjected to immunofluorescence of tdTomato and insulin. The examined islets of individual mice are plotted as circles, which area represents the number of insulin⁺ cells in the islet, and the center position marks the percentage of Tomato⁺ cells in the insulin⁺ cell population.