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. 2016 May 10;5(7):437–448. doi: 10.1016/j.molmet.2016.05.001

Figure 3.

Figure 3

Active β-to-α conversion in diet-induced obese mice. [MIP-rtTA; TRE-Cre; Rosa26-Tomato; PANIC-ATTAC] mice were subjected to 1-week of doxycycline diet and 1-week washout. Subsequently, mice were subjected to no challenge, an ATTAC challenge, multiple pregnancies, or high-fat diet-induced obesity. (A) Immunofluorescence of tdTomato (red) and glucagon (green) on pancreas sections. Top panels: glucagon signal only. Bottom panels: Tomato merged with glucagon and DAPI (blue). Arrowheads: Tomato+glucagon+ cells (yellow). Insets: representative Tomato+glucagon+ cells in the boxed area. Scale bar: 100 μm. (B and C) In each mouse from (A), the total numbers of Tomato+glucagon+ cells were counted, and calculated for percentage in the population of Tomato+ (B) or glucagon+ (C) cells. Data are presented as mean ± SEM. Dots: individual mice. **P < 0.01 versus the no challenge control, by unpaired t-test.