Table 1.

Primers’ sequences used to quantify mRNA expression.

Gene	Seq forward	Seq reverse
Igf-1	GTGAGCCAAAGACACACCCA	ACCTCTGATTTTCCGAGTTGC
Igf-1R	CTCTGTTACCTCTCCACCAT	CTTCTCACACATGGGCTTCT
Irs-1	CTCAGTCCCAACCATAACCAG	GAGTGTTCATAGGCGAGATGG
Rheb	CGA TCC AAC CAT AGA GAA CAC	AAT ATT CAT CCT GCC CCG CT
Mtor	TGC CGC TGA GAG ATG ACA ATG	GTT GTT AAT GCT GAT GAG GG
Rictor	TCG GGG TTC GTG GTT CAT TA	GTC CTG TTT TGT TCC ACT GC
Rptor	ACC TGT TCA CAT CCT GCC TCA	GGC CAG GGA TCT TTT CTA TC
Myod1	GCCCGCGCTCCAACTGCTCTGAT	CCTACGGTGGTGCGCCCTCTGC
Myf5	TGACGGCATGCCTGAATGT	GCTGGACAAGCAATCCAAGC
Fst	AAAACCTACCGCAACGAATG	GGTCTGATCCACCACACAAG
Fstl1	AATGGCAAGACCTACCTCAACC	GTGCCCATCATAATCAACCTGG
Mstn	GCAAAATTGGCTCAAACAGCC	AGGGATTCAGCCCATCTTCTC
Hdac4	TGAGAGTGAGGAGGAAGAAGCG	CAAATGACACAGGGATGCCAG

Igf-1, insulin-like growth factor 1; Igf-1R, insulin-like growth factor 1 receptor; Irs-1, insulin receptor substrate-1; Rheb, Ras homolog enriched in brain; Mtor, mechanistic target of rapamycin (serine/threonine kinase); Rptor, regulatory-associated protein of mTOR complex 1; Rictor, independent companion of MTOR complex 2; Myod1, myogenic differentiation1; Myf5, myogenic factor 5; Fst, follistatin; Fstl1, follistatin-like 1; Mstn, myostatin; Hdac4, histone deacetylase 4.