Figure 2.
Ablation of IR with Adipoq-Cre impairs adipose tissue function and development. (A) Representative images from H&E staining of iWAT sections of 6-wk-old male WT or IRFKO mice. (B) Distribution of adipocyte size of iWAT adipocytes as determined by histomorphometric analyses (n = 5). Data presented are means ± SD. Statistical significance determined by two-way ANOVA with Sidak's multiple comparisons test. *p < 0.05. Serum leptin (C) and adiponectin levels of 6- or 12-wk-old male or female WT or KO mice (n = 5–7). (D) Leptin and adiponectin mRNA levels in iWAT from 6-wk-old male or female WT or KO mice (n = 5). (E) Expression of differentiation markers in iWAT from 6-wk-old male or female WT or KO mice (n = 5). All qPCR data are normalized to TBP and all quantitative data presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.