Figure 1.

Late fetuses and newborn Agpat2^−/−mice have normal adipose tissue mass, anatomical distribution and Perilipin-1 expression. (A) Oil red O/hematoxylin stained transversal thoracic sections revealed normal distribution of adipose tissue in E18.5 fetuses and newborn Agpat2^+/+ and Agpat2^−/− mice. (B) Higher magnification of the dorsal areas stained with Oil red O showed a thin layer of lipid-laden cells in the hypodermis corresponding to developing subcutaneous white adipose tissue (scWAT) in animals of both genotypes. scWAT is separated from the interscapular brown adipose tissue (iBAT) by the panniculus carnosus muscle (outlined by dashed line) (C) Perilipin-1 immunofluorescence staining (green) revealed that lipid-laden cells correspond to adipocytes expressing Perilipin-1. F-actin was detected with Phalloidin/rhodamine (red), indicating skeletal muscle. Nuclei were stained with DAPI (blue). Images are representative of 6 embryos or fetuses per genotype and age.