Supplementary Figure 1.

(A) Oil red O/hematoxylin stained transversal sections at the inguinal level of newborn Agpat2^−/− mice. Bilateral subcutaneous inguinal WAT depots (left and middle images) are located between the skin and muscle fascia just anterior to the lower segment of the hind limbs. Higher magnification of the inguinal WAT depots stained with Oil red O (right image). (B) Perilipin-1 immunofluorescence staining (green) of inguinal WAT. F-actin was detected with phalloidin/rhodamine (red), indicating skeletal muscle. Nuclei were stained with DAPI (blue). (C) Development of hepatic steatosis matches adipose tissue destruction in Agpat2^−/− mice. Histological analysis (hematoxylin-eosin staining) shows growing lipids build up in the liver of Agpat2^−/− mice starting at P2.5. No differences between female and male Agpat2^−/− mice were detected.