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(A) Immunoblot analysis of whole-cell protein extracts from Agpat2+/+ and Agpat2−/− MEFs at different days of differentiation. 50 μg of proteins were loaded, total Akt was used as loading control. (B) At the indicated times, the number of lipid-laden cells were counted as previously described. Graph shows the percentage of lipid-laden cells. (C) TUNEL assay in 10 days differentiated MEFs.
