Table 3. Association of rs8102595 and rs3826795 with anthropometric and metabolic characteristics, mRNA expression and DNA methylation.
| rs8102595 |
rs3826795 |
|||||
|---|---|---|---|---|---|---|
| A/A | A/G + G/G | p-value | A/A+ A/G | G/G | p-value | |
| N | 446 | 95 | 208 | 336 | ||
| Men/Women | 151/295 | 32/63 | 73/135 | 110/226 | ||
| Age | 52.83 ± 15.79 | 55.48 ± 15.44 | 0.482 | 49.56 ± 15.31 | 50.72 ± 14.69 | 0.278 |
| BMI (kg/m2) | 43.48 ± 13.74 | 42.51 ± 13.50 | 0.239 | 43.64 ± 14.04 | 42.93 ± 13.32 | 0.908 |
| Body weight (kg) | 126.86 ± 42.81 | 124.57 ± 40.14 | 0.680 | 128.42 ± 45.54 | 124.60 ± 41.15 | 0.769 |
| Height (m) | 1.69 ± 0.09 | 1.69 ± 0.9 | 0.628 | 1.69 ± 0.09 | 1.69 ± 0.09 | 0.763 |
| Waist (cm) | 124.26 ± 29.98 | 121.84 ± 30.09 | 0.798 | 124.46 ± 30.43 | 122.85 ± 29.87 | 0.935 |
| Hip (cm) | 130.53 ± 28.99 | 128.59 ± 28.38 | 0.851 | 129.54 ± 28.38 | 130.08 ± 29.56 | 0.676 |
| WHR | 0.95 ± 0.13 | 0.96 ± 0.16 | 0.316 | 0.96 ± 0.16 | 0.94 ± 0.12 | 0.921 |
| VAT area (cm2) | 242.93 ± 173.84 | 237.02 ± 159.92 | 0.575 | 256.05 ± 183.40 | 228.98 ± 159.97 | 0.674 |
| SAT area (cm2) | 1095.74 ± 795.48 | 1129.73 ± 819.78 | 0.536 | 1122.85 ± 774.80 | 1094.46 ± 817.64 | 0.902 |
| VAT mean | 123.00 ± 20.82 | 122.08 ± 20.60 | 0.999 | 119.69 ± 25.71 | 124.66 ± 17.25 | 0.014 |
| SAT mean | 127.37 ± 19.89 | 127.51 ± 17.42 | 0.486 | 126.50 ± 19.04 | 127.99 ± 19.84 | 0.334 |
| VAT max | 209.23 ± 58.51 | 230.21 ± 96.06 | 0.060 | 210.73 ± 74.84 | 213.66 ± 63.47 | 0.109 |
| SAT max | 214.28 ± 70.88 | 249.22 ± 110.69 | 1.23 × 10−3 | 224.71 ± 80.22 | 217.94 ± 79.94 | 0.987 |
| CT ratio (vis/sc) | 0.47 ± 0.63 | 0.38 ± 0.30 | 0.922 | 0.40 ± 0.42 | 0.48 ± 0.66 | 0.826 |
| Body fat (%) | 41.95 ± 11.35 | 42.26 ± 11.72 | 0.496 | 41.15 ± 11.88 | 42.57 ± 11.11 | 0.607 |
| CRP (mg/dl) | 12.04 ± 15.09 | 11.20 ± 16.05 | 0.935 | 13.09 ± 15.67 | 11.34 ± 15.49 | 0.198 |
| Leucocytes/nl | 8.21 ± 2.88 | 8.08 ± 2.50 | 0.743 | 8.42 ± 3.22 | 8.00 ± 2.48 | 0.155 |
| Blood Met (%) | 20.99 ± 8.07 | 22.31 ± 5.11 | 0.143 | 21.43 ± 7.36 | 21.27 ± 7.56 | 0.811 |
| Met SAT (%) | 11.95 ± 5.86 | 16.34 ± 6.54 | 0.011 | 13.56 ± 7.38 | 12.69 ± 5.83 | 0.784 |
| Met VAT (%) | 17.04 ± 5.61 | 19.69 ± 6.10 | 0.038 | 18.20 ± 4.41 | 17.46 ± 6.18 | 0.401 |
| SAT HIF3A mRNA | 21.08 ± 72.62 | 7.43 ± 40.53 | 0.209 | 11.58 ± 49.82 | 22.60 ± 76.47 | 0.660 |
| VAT HIF3A mRNA | 23.92 ± 106.19 | 10.45 ± 50.03 | 0.073 | 16.80 ± 82.25 | 24.09 ± 106.69 | 0.729 |
Due to the low minor allele frequency (MAF) of the studied polymorphisms, subjects homozygous for the minor alleles (n = 3 for rs8102595, n = 16 for rs3826795) were combined with heterozygous groups (i.e. dominant mode of inheritance was used for statistical analyses).
p-value adjusted for age, gender and BMI and diabetes status; BMI – Body Mass Index, WHR – waist-to-hip ratio, SAT – subcutaneous adipose tissue, VAT- visceral adipose tissue CRP – C-reactive protein, Met Blood (%)/Met SAT (%)/Met VAT (%) - Methylation of cg22891070 in HIF3A in blood/SAT/VAT, HIF3A mRNA – mRNA expression of HIF3A in subcutaneous/visceral adipose tissue.