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. 2016 Jun 27;6:28673. doi: 10.1038/srep28673

Figure 5. Impact of BcMtg2 deletion on cell wall lyase sensitivity and sclerotial formation.

Figure 5

(A) Comparison of protoplast among B05.10, ΔBcMtg2 and ΔBcMtg2C After mycelia incubated at 30 °C for 45 min in 1.5% lyase buffer. (B) The number of protoplast release by each strain on lyase buffer. Bars in each column denote the standard errors of three experiments. Values on the bars followed by the same letter are not significantly different at P = 0.05. (C) Comparison of sclerotia formation among B05.10, ΔBcMtg2 and ΔBcMtg2C after cultivated on CM and PDA medium for 4 weeks at 25 °C in darkness. (D) The number of sclerotia was counted on both CM and PDA. Bars denote standard errors from three experiments. Values on the bars followed by the same letter are not significantly different at P = 0.05.