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. 2016 Jun 27;6:28402. doi: 10.1038/srep28402

Figure 4. Effects of SLC26A3 and SLC26A6 inhibitors on embryo cleavage and HCO3−-dependent signalling cascade.

Figure 4

(A–F) Effects of inhibiting CFTR (CFTRinh172, 10 μM), SLC26A3 (niflumate, 20 μM) and SLC26A6 (DIDS, 20 μM) on preimplantation embryo cleavage in complete TALP (A,B) control: 32/38 embryos; CFTRinh172: 6/34 embryos; niflumate: 9/35 embryos; DIDS: 14/40 embryos), HCO3−-deficient TALP (C,D) control: 5/30 embryos; CFTRinh172: 7/31 embryos; niflumate: 4/32 embryos; DIDS: 6/31 embryos) and Cl−-deficient TALP (E,F, control: 6/32 embryos; CFTRinh172: 4/31 embryos; niflumate: 5/30 embryos; DIDS: 6/33 embryos). Scale bar: 100 μm. Summary of the results are shown on the right panel (B,D,F). ns indicates P > 0.05, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001 (by one-way ANOVA, n = 4). (G–I) Quantitative real-time PCR showing the expression of miRNA-125b (G), p53 (H) and p21 (I) after CFTRinh172, niflumate or DIDS treatment. * indicates P < 0.05, ** indicates P < 0.01 (by one-way ANOVA, ~100 embryos in each experiment, n = 4).