Table 1. Dimensions of replicated rosette CSCs and individual lobes measured on original images and class averages.
| Type of image analyzed | Geometry | Perimeter (nm) | Diameter (nm) | Lobe Area (nm2) |
|---|---|---|---|---|
| Original images (n = 324) | Circle | 78.4 ± 4.9 | 24.9 ± 1.6 | 39.9 ± 6.5 (n = 300) |
| Hexagon | 74.8 ± 4.5 | 21.4 ± 1.3 | ||
| Class averages of entire rosette CSCs | ||||
| ISAC/SPARX (n = 6) | Circle | 76.8 ± 2.5 | 24.3 ± 0.8 | 37.6 ± 3.3 (n = 36) |
| Hexagon | 78.0 ± 3.1 | 22.5 ± 0.9 | ||
| RELION (n = 2) | Circle | 78.2 ± 0.4 | 24.9 ± 0.1 | 42.0 ± 2.7 (n = 12) |
| Hexagon | 79.9 ± 1.5 | 23.1 ± 0.4 | ||
| EMAN2 (n = 6) | Circle | 76.4 ± 2.3 | 24.3 ± 0.7 | 36.7 ± 4.9 (n = 36) |
| Hexagon | 78.7 ± 1.9 | 22.7 ± 0.5 | ||
| Class averages of individual lobes | ||||
| 6 classes | – | – | – | 40.6 ± 4.4 (n = 6) |
| 12 classes | – | – | – | 38.7 ± 2.9 (n = 12) |
| Means | ||||
| Means for Original Images | 76.6 | 23.1 | 39.9 | |
| Means for Class Averages | 78.0 | 23.6 | 39.1 | |
| Overall Means | 77.3 | 23.4 | 39.5 |
Diameter was estimated from the perimeter using two geometries. ‘Lobe Area’ is the average value for one lobe. ± values indicate standard deviation; n values for perimeter and diameter are shown in column 1 and indicate entire rosette CSCs; n values for lobe area are shown in column 5 and indicate individual lobes or lobe image averages measured.