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. 2016 Jun 27;6:28684. doi: 10.1038/srep28684

Figure 8. Ndk regulates the expression of the ExsA-mediated T3S regulon and exoproducts through the QS system.

Figure 8

PAO1 and Δndk strains were inoculated into LB broth at an initial OD600 of 0.01. Exogenous 3-oxo-C12-HSL, C4-HSL or PQS (4 μg/ml or 40 μg/ml) was added to the culture medium of Δndk cells. The bacteria and culture supernatants were harvested for subsequent analysis at the indicated culture time. (a) Real-time PCR analysis for T3SS-encoding gene expression (signal molecules at 40 μg/ml, 12 h). (b) Western blot analysis for the intracellular expression of T3SS proteins (signal molecules at 40 μg/ml, 12 h). (c) Skim milk plate and ECR assays for secreted proteases (12 h). (d) Analysis for pyocyanin production (12 h). (e) Real-time PCR analysis for exoproduct-related gene expression (signal molecules at 40 μg/ml, 12 h). C12 indicate 3-oxo-C12-HSL. C4 indicates C4-HSL. Data represent mean ± SD from three independent experiments. *Indicates P < 0.05 compared to PAO1 strain. #Indicates P < 0.05 compared to Δndk strain.