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. 2016 Jun 27;6:26935. doi: 10.1038/srep26935

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Copyright © 2016, Macmillan Publishers Limited

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RBCs were incubated with DAF-FM (5 μm) for 30 minutes, and subjected to different physical forces using centrifuge, shaker, rocker or vortex. A dose-dependent increase in NO production was observed in RBCs subjected to (a) vortex (200, 400, 600, 800 rpm), followed by decreased NO production at higher speeds (1000, 1200 rpm) (n = 3; *p < 0.001). Significant increase in NO production was observed in RBCs subjected to (b) centrifugation (3000, 4500 rpm) (n = 3; *p < 0.038), (c) rocker (20, 40, 80 rpm) (n = 3; *p < 0.001), and (d) shaker (50, 100, 200 rpm) (n = 3; *p < 0.004), versus control RBC (static).