Skip to main content
. 2016 Jun 27;6:26935. doi: 10.1038/srep26935

Figure 2. Chemical agonists and vortexing induce comparable levels of NO production by RBCs.

Figure 2

RBCs were pre- incubated with insulin (5 μM), calcium chloride (1 mM) or L-arginine (1 mM) for 30 mins at 37 °C, followed by incubation with DAF-FM (5 μm) for 30 minutes prior to measurement of NO. RBCs treated with agonists showed a significant 2–3 fold increase in NO production versus untreated controls (*p < 0.042). A similar increase in NO was observed in vortexed RBC (800 rpm for 20 seconds) versus static controls (n = 3;*p < 0.001).