Figure 3. Measurement of NO in Vortexed RBC with Ultrasensitive NO electrode.

Real time NO measurement in a suspension of RBCs (6 × 10⁵ RBC/mL) was carried out using an optically isolated, multi-channel free radical analyzer with an NO-selective membrane (Apollo 4000). (a) Vortexed RBC produced 108.415 pMoles of NO, whereas static RBC produced 10.19 pMoles of NO (n = 3; *p = 0.035, t-test). (b) Recording showing NO measurements from static and vortexed RBCs from same donor. (c) RBC-NO level recorded by continuous vortex mode. Real time NO measurement in a suspension of RBCs (1 × 10⁶ RBC/mL) was carried out using the NO-selective membrane. After stabilization with buffer, the instrument was subjected to sustained vortex in buffer (800 rpm) until it re-stabilized. At 2000 seconds, RBCs were added and vortexed, and the instrument re-stabilized for an additional 600 seconds. Vortexed RBCs continuously produced NO from 600 to 708 seconds (108 sec). In the first 20 secs, 5.98 μM NO were produced. A total of 6.07 μM NO were produced from 600 to 708 seconds (108 sec), (n = 3).