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. 2016 Jun 27;6:28750. doi: 10.1038/srep28750

Figure 2. Differential c-Fos expression patterns in netrin-G1 gKO mice after fear conditioning.

Figure 2

(a,c,e) Representative images of hippocampus, cingulate cortex, and amygdala from WT mice. (b,d,f) Representative images of the corresponding brain regions from netrin-G1 gKO. (g) The number of c-Fos positive cells in each ROI was compared between WT and netrin-G1 gKO mice using Student’s two-tailed t test, n = 8, (4 animals × 2 sections) per genotype. Netrin-G1 gKO mice had significantly fewer c-Fos-positive cells in CA1 compared with WT mice (p < 0.01), while there was no significant difference in the CA3 (p = 0.62), DG (p = 0.44) and Cg (p = 0.31). In the amygdala, the number of c-Fos positive cells was markedly decreased in almost all subnuclei of the amygdala, including the BL (p < 0.001), BM (p < 0.001), LA (p < 0.001), CeM (p < 0.001), and Me (p = 0.03). In the CeL, however, there was no significant difference between genotypes (p = 0.31). Black columns represent WT and red columns represent gKO, and the data are represented as the mean + SEM. *p < 0.05, **p < 0.01, ***p < 0.001; scale bar: 500 μm (a,b); 250 μm (c,d); 280 μm (e,f).