Table 1. Endothelial cells are the main targets of DEET.
| Proliferation (% of control ± SEM) | DEET 10−8 M | DEET 10−5 M | pFHHSiD 10−7 M DEET 10−8 M | pFHHSiD 10−7 M DEET 10−5 M |
|---|---|---|---|---|
| U87MG | 98.21 ± 1.59 | 97.18 ± 1.28 | / | / |
| HUVEC | 113.55 ± 3.16* | 112.03 ± 2.90* | 102.79 ± 5.41 | 100.82 ± 5.23 |
CyQUANT assay reveals that both concentrations of DEET (10−8 M and 10−5 M) do not increase proliferation of U87MG cell line while increasing HUVEC one after 24 h treatment. The selective M3 muscarinic receptor antagonist pFHHSiD prevents the DEET-induced endothelial proliferation. Results are expressed at mean ± SEM; *p < 0.05 compared to control (Kruskal-Wallis with Dunn’s multiple comparison test).